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moi oc43 coronavirus  (ATCC)


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    ATCC moi oc43 coronavirus
    Recombinant proteins expressed in E.coli.
    Moi Oc43 Coronavirus, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/moi+oc43+coronavirus/Betacoronavirus+1/pmc08096524-71-6-11
    Average 98 stars, based on 458 article reviews
    moi oc43 coronavirus - by Bioz Stars, 2026-09
    98/100 stars

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    1) Product Images from "Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody"

    Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody

    Journal: Virus Research

    doi: 10.1016/j.virusres.2021.198445

    Recombinant proteins expressed in E.coli.
    Figure Legend Snippet: Recombinant proteins expressed in E.coli.

    Techniques Used: Recombinant, Plasmid Preparation, Sequencing

    Characterization of RAb recognizing human CoV NPs. (A) Coomassie-stained SDS-PAGE of purified NPs of seven human CoVs. (B) and (C) Western blot and ELISA analysis of RAb cross-reacting with NPs of seven human CoVs. M: protein ladder marker. (D) Immunocytochemistry analysis of RAb detecting Ad293 cells transfected with pcDNA-SARS-CoV-2-NP, and HRT-8 cells infected with CoV OC43. Mouse anti-OC43-RBD and anti-PBS sera were used as the controls.
    Figure Legend Snippet: Characterization of RAb recognizing human CoV NPs. (A) Coomassie-stained SDS-PAGE of purified NPs of seven human CoVs. (B) and (C) Western blot and ELISA analysis of RAb cross-reacting with NPs of seven human CoVs. M: protein ladder marker. (D) Immunocytochemistry analysis of RAb detecting Ad293 cells transfected with pcDNA-SARS-CoV-2-NP, and HRT-8 cells infected with CoV OC43. Mouse anti-OC43-RBD and anti-PBS sera were used as the controls.

    Techniques Used: Staining, SDS Page, Purification, Western Blot, Enzyme-linked Immunosorbent Assay, Marker, Immunocytochemistry, Transfection, Infection

    Quantification of SARS-CoV-2 NP by double-antibody sandwich ELISA. (A) Double-antibody sandwich ELISA was established. The plates were coated with RAb, then incubated with purified SARS-CoV-2-NP protein under increasing concentrations. Mouse antiserum against SARS-CoV-2-NP and HRP-conjugated secondary antibody was then added in turn. (B) Double-antibody sandwich ELISA was used to quantify SARS-CoV-2-NP expressed in Ad293 cells. Purified SARS-CoV-2-NP (2 μg/mL) was used as the positive control, and Ad293 cellular supernatant was used as the negative control. HAdV-7, RSV, InfA, HCoV-OC43, and HCoV-229E could not be detected by this sandwich ELISA.
    Figure Legend Snippet: Quantification of SARS-CoV-2 NP by double-antibody sandwich ELISA. (A) Double-antibody sandwich ELISA was established. The plates were coated with RAb, then incubated with purified SARS-CoV-2-NP protein under increasing concentrations. Mouse antiserum against SARS-CoV-2-NP and HRP-conjugated secondary antibody was then added in turn. (B) Double-antibody sandwich ELISA was used to quantify SARS-CoV-2-NP expressed in Ad293 cells. Purified SARS-CoV-2-NP (2 μg/mL) was used as the positive control, and Ad293 cellular supernatant was used as the negative control. HAdV-7, RSV, InfA, HCoV-OC43, and HCoV-229E could not be detected by this sandwich ELISA.

    Techniques Used: Sandwich ELISA, Incubation, Purification, Positive Control, Negative Control

    Related Articles

    Infection:

    Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody
    Article Snippet: The HCT-8 cells were obtained from ATCC (CCL-244) and maintained in 1640 medium (Gibco, USA) supplemented with 100 IU/mL penicillin, 100 mg/mL streptomycin, 1% Non-essential amino acids, 25 mM Hepes, as well as 10 % fetal bovine serum. .. HRT-8 cells were infected with 0.01 MOI OC43 coronavirus obtained from ATCC (VR-1558) in 24-well plates and then received the 4-day culture at 33 °C in a humidified 5% CO 2 atmosphere. ..



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    moi oc43 coronavirus  (ATCC)
    98
    ATCC moi oc43 coronavirus
    Recombinant proteins expressed in E.coli.
    Moi Oc43 Coronavirus, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/moi+oc43+coronavirus/Betacoronavirus+1/pmc08096524-71-6-11
    Average 98 stars, based on 1 article reviews
    moi oc43 coronavirus - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier

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    Recombinant proteins expressed in E.coli.

    Journal: Virus Research

    Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody

    doi: 10.1016/j.virusres.2021.198445

    Figure Lengend Snippet: Recombinant proteins expressed in E.coli.

    Article Snippet: HRT-8 cells were infected with 0.01 MOI OC43 coronavirus obtained from ATCC (VR-1558) in 24-well plates and then received the 4-day culture at 33 °C in a humidified 5% CO 2 atmosphere.

    Techniques: Recombinant, Plasmid Preparation, Sequencing

    Characterization of RAb recognizing human CoV NPs. (A) Coomassie-stained SDS-PAGE of purified NPs of seven human CoVs. (B) and (C) Western blot and ELISA analysis of RAb cross-reacting with NPs of seven human CoVs. M: protein ladder marker. (D) Immunocytochemistry analysis of RAb detecting Ad293 cells transfected with pcDNA-SARS-CoV-2-NP, and HRT-8 cells infected with CoV OC43. Mouse anti-OC43-RBD and anti-PBS sera were used as the controls.

    Journal: Virus Research

    Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody

    doi: 10.1016/j.virusres.2021.198445

    Figure Lengend Snippet: Characterization of RAb recognizing human CoV NPs. (A) Coomassie-stained SDS-PAGE of purified NPs of seven human CoVs. (B) and (C) Western blot and ELISA analysis of RAb cross-reacting with NPs of seven human CoVs. M: protein ladder marker. (D) Immunocytochemistry analysis of RAb detecting Ad293 cells transfected with pcDNA-SARS-CoV-2-NP, and HRT-8 cells infected with CoV OC43. Mouse anti-OC43-RBD and anti-PBS sera were used as the controls.

    Article Snippet: HRT-8 cells were infected with 0.01 MOI OC43 coronavirus obtained from ATCC (VR-1558) in 24-well plates and then received the 4-day culture at 33 °C in a humidified 5% CO 2 atmosphere.

    Techniques: Staining, SDS Page, Purification, Western Blot, Enzyme-linked Immunosorbent Assay, Marker, Immunocytochemistry, Transfection, Infection

    Quantification of SARS-CoV-2 NP by double-antibody sandwich ELISA. (A) Double-antibody sandwich ELISA was established. The plates were coated with RAb, then incubated with purified SARS-CoV-2-NP protein under increasing concentrations. Mouse antiserum against SARS-CoV-2-NP and HRP-conjugated secondary antibody was then added in turn. (B) Double-antibody sandwich ELISA was used to quantify SARS-CoV-2-NP expressed in Ad293 cells. Purified SARS-CoV-2-NP (2 μg/mL) was used as the positive control, and Ad293 cellular supernatant was used as the negative control. HAdV-7, RSV, InfA, HCoV-OC43, and HCoV-229E could not be detected by this sandwich ELISA.

    Journal: Virus Research

    Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody

    doi: 10.1016/j.virusres.2021.198445

    Figure Lengend Snippet: Quantification of SARS-CoV-2 NP by double-antibody sandwich ELISA. (A) Double-antibody sandwich ELISA was established. The plates were coated with RAb, then incubated with purified SARS-CoV-2-NP protein under increasing concentrations. Mouse antiserum against SARS-CoV-2-NP and HRP-conjugated secondary antibody was then added in turn. (B) Double-antibody sandwich ELISA was used to quantify SARS-CoV-2-NP expressed in Ad293 cells. Purified SARS-CoV-2-NP (2 μg/mL) was used as the positive control, and Ad293 cellular supernatant was used as the negative control. HAdV-7, RSV, InfA, HCoV-OC43, and HCoV-229E could not be detected by this sandwich ELISA.

    Article Snippet: HRT-8 cells were infected with 0.01 MOI OC43 coronavirus obtained from ATCC (VR-1558) in 24-well plates and then received the 4-day culture at 33 °C in a humidified 5% CO 2 atmosphere.

    Techniques: Sandwich ELISA, Incubation, Purification, Positive Control, Negative Control